Plant extracts with biofilm inhibitory activity biofilms are the default mode of life for many bacterial species and biofilmbased infections cause harm to millions of humans annually. In vitro antifungal activity of dichloromethane and methanol extracts mgml. Studies on the antimicrobial activity of essential oils from t. A study of antimicrobial activity of few medicinal herbs. Antimicrobial activity of crude extracts of three medicinal. Pdf antimicrobial activities of various medicinal and commercial. The antimicrobial potential of the above plant extracts was seen against the test organism using agar gel diffusion susceptibility test by standard technique of opara and anasa19939. Antimicrobial activity, toxicity and phytochemical screening. The extract of three medicinal plants used in nigerian folk medicine showed a highly antibacterial activity against some food borne pathogens. Phytochemical analysis of phyllanthus amarus extracts s.
Pdf antimicrobial activity of medicinal plant leaf. Pdf selected cameroonian plants have been investigated for their antifungal and antibacterial activity against five species. Introduction even though pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by microorganisms has increased. Results of antimicrobial activity of the five plant extracts can suggested that s. All extracts were stored at 4c till further analysis. Aug 20, 20 in addition to antioxidant activity, plant phenolic compounds, such as flavonoids and tannins, have been shown to possess antimicrobial activities 1618. Antibacterial activity of aquilaria crassna leaf extract.
The effect of various plant extracts was measured on several important oxidation indicators. Antibacterial activities of the extracts were first screened by agarwell diffusion method as described previously. Antimicrobial activity of medicinal plant extracts and. In relation to tetracycline activity, ethanol and ethyl acetate extracts showed similar or same activities while the water activity was higher than standard 100. Antimicrobial potential of some plant extracts against. Screening of antibacterial activity of the medicinal plant. Microorganisms dichloromethane extracts methanol extracts ac mp sc ta ro pg ac mp sc ta ro pg. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Feb 27, 2016 antimicrobial activity of various plants extracts 1.
Antimicrobial activity of single and combined extracts of. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. Screening of in vitro antimicrobial activity of plants used in traditional. Feb 09, 2015 the observed mic and mbc values of the plant extracts in this study are in a range or lower than the values of other plant extracts reported from different countries 15, but are higher than those values that have been reported in a number of reports 11, 16, 17. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. The highest antibacterial activity of crude eos was observed in p. Antibacterial activity the agar well diffusion method was performed to exploit antibacterial potential of used extracts 8. Studies on in vitro antimicrobial activity of ethanol extracts of rauvolfia tetraphylla. All in all, data about specific antibiotic properties of coumarins are. Mic value shows that these plant extracts have the least antimicrobial activity. Species aegopodium podagraria, daucus carota, heracleum sphondylium and pimpinella. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Antimicrobial activity of ethanol leaf extracts of. Among the four extracts of neem, petroleum ether and chloroform extract showed strong antimicrobial activity against s.
The mic testing was performed for all the plant extracts that were judged as active inhibition zone 7 mm against at least one test organism by agarwell diffusion method. Antimicrobial activity of aqueous and organic extracts. Association of antibiotics and plant extracts showed synergistic antibacterial. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Ates and others published antimicrobial activities of various medicinal and commercial plant extract find, read and cite all the. Chemical composition and antibacterial activity of essential. Antimicrobial activity of some important medicinal plant. The results indicated that the methanol extracts of o. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. The antimicrobial compounds produced by plants are active against plant and human pathogenic microorganisms. Punica granatum, citrullus colocynthes, curcuma long, albizia lebbeckandazardirachta indicaare used in traditional medical practice in sudan for the treatment of gastrointestinal tract infections. Journal of biologically active products from nature. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus. Assessment of antioxidant activity of plant extracts by.
Antimicrobial activity of selected medicinal plants against. Dried leaves of plants were mechanically ground, and 2 g of plant was extracted with 20 ml of acetone or chloroform or methanol, then it was gently heated. The main objectives of this study were to examine antibacterial activity of the aquilaria crassna leaf extract against staphylococcus epidermidis and its underlying mechanism. General antimicrobial activity was documented in woodruff galium odoratum extracts 224. Antiplasmodial, antimicrobial and cytotoxic activities of extracts from selected medicinal plants growing in tanzania.
We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of nepal against clinical and 2 reference bacterial. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Figure 3 minimal inhibitory concentration mic of plant extractsphytochemicals against antibiotic resistant bacteria.
Many methods for detecting such activity are available, but since they are not equally sensitive or even based on the same. Evaluation of antibacterial activity of some medicinal plants. Antimicrobial activity of some important medicinal plant against plant and human pathogens 12b. In vitro antimicrobial activity of some medicinal plants. Antibacterial activity of plant extracts of alstonia scholaris. All plant extracts methanol and aqueous show significant antimicrobial activity against both test bacteria s. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. Pdf antimicrobial activity of single and combined extracts of. The high temperature during soxhlet extraction in our study might be. None of the aqueous extracts showed antimicrobial activity. Abts, dpph and frap assays are widely used to determine the antioxidant capacity in plant extracts because of their simplicity, stability and accuracy. Satish 1department of studies in applied botany and biotechnology, university of mysore, manasagangotri, mysore570 006 2herbal drug technology laboratory, department of.
Different parts of the plants were collected, air dried, and powdered in a homogenizer, and 10 g of each plant was used for aqueous and ethanol extraction. The most active plant was torilis anthriscus especially in relation to pseudomonas glycinea. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Jun 03, 2015 evaluation of antibacterial activity of some medicinal plants used in sudanese folk medicine for treatment of gastrointestinal tract infections. The study of antioxidant and antimicrobial activity of. Fresh leaves of eleven different plant species free from. Antimicrobial activity of wild olive crude extracts in vitro. Antibacterial activity of some plant extracts nihal dog ru oz 1, zuhal zeybek 1, ali karagoz 2 1 istanbul university, faculty of science, department of b iology, 344, vezneciler, istanbultu.
Antibacterial activities of plant extracts were compared with those of standard antibiotics. Biochemistry graduates to have results from their final year. About 250,000 living plant species contain a much greater diversity of bioactive compounds than any chemical library made by humans but only few plant species have been systematically investigated for the presence of bioactive compounds wakdikar, 2004. Antimicrobial activity of some indian medicinal plants. The results showed that extracts of mint, lemon balm and coriander among others inhibited the growth of e. The methanol and aqueous extracts from different plant parts peel of fruits, rhizome, fruits, leaves, and gum respectively at a concentration of 100 mgml, were evaluated against 20 clinical.
Aquilaria crassna pierre ex lecomte has been traditionally used in thailand for treatment of infectious diseases such as diarrhoea and skin diseases for a long time. Antibacterial and antifungal activities of thespesia. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Antimicrobial activity, toxicity and phytochemical. Boiled water extract of neem leaf showed moderate zone of inhibition against escherichia coli 10 mm and. Antimicrobial activity of medicinal plant leaf extracts.
Antimicrobial activity of five herbal extracts against multi drug. The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of ocimum basilicum l. Biological screening of various medicinal plant extracts. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Upon heating, it was rinsed for 24 h at room temperature. The methanolic extract of caryophyllus aromaticus presented the highest antis. Antimicrobial activity of medicinal plants against human pathogenic bacteria 657 inflammatory properties of plants mahesh and satish, 2008.
The activity of crude essential oils against the three bacteria isolates was within the extremely sensitive category diameters of the inhibition zone larger than 20 mm after 24 and 48 hours of incubation table 1. Four different solvents were used for the extraction. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. Boiled water extracts of guava leaf showed the largest zone of inhibition 22 mm against v. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material. Minimum inhibitory concentration mic determination.
Chemical composition and antibacterial activity of. In vitro investigation of antimicrobial activities of. Antimicrobial activity of selected medicinal plants. Antibacterial activity of aqueous extract of calotropis. Antimicrobial activity of various extracts of ocimum. Antimicrobial activity of medicinal plants against human. Studies to determine the antibacterial acitivities of. Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial. Antibacterial and antifungal activities of thespesia populnea.
The study of antioxidant and antimicrobial activity of extracts for meat marinades 9690 romanian biotechnological letters, vol. Satish 1department of studies in applied botany and biotechnology, university of mysore, manasagangotri, mysore570 006 2herbal drug technology laboratory, department of studies in microbiology. Issn phytochemical and antimicrobial evaluation of plant. Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1. In this activity, the students will be testing the effectiveness of several types of plant extract antibiotics, which they have produced from plants selected from their local ecosystem. In this study, nine plants extracts were screened for potential antibacterial activity table. The three bacteria used in the current study were all gramnegative. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Studies on some plant extracts for their antimicrobial. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c.
The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Antimicrobial activity of plant extract was considered to be good if its mic was less than 100. Table 2 mic of plant extracts against the microorganisms by micro dilution broth assay. Methanol extracts of all plants were more active than aqueous extracts. Water extracts of green tea leaf at boiling and room temperature showed 17. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. Antimicrobial activity of crude extracts of some plant leaves. Plant extracts since many of the essential oils inhibited the growth of e. Evaluation of antibacterial activity of some traditionally. It was found that most plant extracts studied had antibacterial and antifungal activities. Antimicrobial activity of crude extracts from plant parts and corresponding calli of f bixa orellana l. Antimicrobial activities of the crude ethanolic extracts of five plants were screened against multidrug resistant mdr strains of.
To prepare plant extracts three different solvents were used. Antimicrobial activity of some plant extracts against bacterial strains. Antimicrobial activity of some plant extracts against. Research article open access antibacterial activity of. Antimicrobial assay the antimicrobial activity of the plant extracts was evaluated using agar well diffusion method 21 with minor modifications. Biochemistry graduates to have results from their final. To determine antibacterial activity of water, oil and methanol extracts of guava psidium guajava, green tea camellia sinensis, neem azadirachta indica and marigold calendula officinalis against different species of bacteria, pseudomonas spp.
Pdf antibacterial activity of medicinal plant extracts. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Bioactive aqueous extracts of twelve yucatecan plants. All the plant extracts were screened for their antimicrobial activity. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens.
Antimicrobial activity of various plants extracts was compared with commercially available antibiotics. Aloe vera, antimicrobial activity, sap, leaves, minimum inhibitory concentration introduction. Research article open access antibacterial activity of some. The methanolic extract of leaf of the plant azadiracta indica, acacia nilotica and witania somnifera showed significant. Increasingly, natural products have potential for the treatment of cancer.
The worldwide increase of multidrug resistance in both community and healthcare associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. Plant extracts were added based on plackettburman design by varying the composition of chosen independent variables. Antiplasmodial, antimicrobial and cytotoxic activities of. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. The present study aimed at evaluating the in vitro antimicrobial activity of plant allium sativum, zingiber officinale, caryophyllus aromaticus, cymbopogon citratus, mikania glomerata and psidium guajava extracts against grampositive and gramnegative bacterial strains isolated from human infections. Antibacterial activities of some borneo plant extracts. The present study shows the antimicrobial activity of the ethanolic extract of the citrus limon peel of 25%, 50%, 75% and 100% had a strong antimicrobial activity against escherichia coli were 15,03 mm. Biological screening of various medicinal plant extracts for. Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants.
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